This reagent kit is designed to detect in clinical material the DNA of pathogens causing urinary tract infections and to identify nucleotide sequences (resistance genes) that determine bacterial resistance to polymyxins, beta-lactam, glycopeptide, and fluoroquinolone antibiotics. The material for the study is DNA preparations obtained directly from urine samples or from cultures of microorganisms grown by plating biological material (urine) on a liquid or solid nutrient medium.
The study allows for the detection in the DNA sample of the following pathogens and antibiotic resistance gene sequences:
DNA of bacteria Proteus mirabilis, Citrobacter spp., Enterobacter spp., Acinetobacter baumannii, Staphylococcus aureus, Staphylococcus saprophiticus, Pseudomonas aeruginosa, Enterococcus faecalis, Streptococcus agalactiae, Streptococcus pyogenes, Klebsiella pneumonia, uropathogenic strains of Escherichia coli (UPEC):
- serogroups O1, O2, O4, O6, O7, O8, O15, O16, O18, O25, O75;
- DNA of the fungal infection pathogen – Candida albicans;
- Sequences of resistance genes to polymyxins, beta-lactam, glycopeptide and fluoroquinolone antibiotics – MCR-1, TEM, SHV, GES, CTX-M-14/15, KPC, VEB, PER, NDM, IMP, VIM, CMY, DHA, OXA-23, OXA-24/40, OXA-48, OXA-51, OXA-58, mecA, vanA/B, qnrB/S.
